Workbeads - Bio-Works
WorkBeads - 20% discount all of March 2019
Immobilized metal-ion affinity chromatography (IMAC) resin
The WorkBeads resins are based on nitrilotriacetic acid (NTA) and the iminodiacetic acid (IDA) chelating groups. The resins can easily be charged before use with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+, Zn2+, Ga3+ or Fe3+. They can then be used for IMAC purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. These resins can also be used for divalent metal ion removal.
- Resin can be charged (or be pre-charged) with the metal ion of choice
- High binding capacity and purity. Outstanding flow properties.
- Reliable and reproducible results
WorkBeads™ 40 TREN resin for multimodal Ion Exchange Chromatography (IEX) has a ligand that is positively charged below approx. pH 9. The resin can be used for several different applications, e.g., for multimodal IEX, for sample cleanup in monoclonal antibody (mAb) purification processes in order to guard the Protein A column from chromatins and other host cell impurities, or as a polishing step in the mAb purification process.
- Improved selectivities through multimodal IEX separation
- Reduced fouling of Protein A resins by chromatin and host cell impurity removal
- High binding capacity and purity
WorkBeads 40 TREN resin contains ligands based on Tris(2-aminoethyl)amine (TAEA). The structure of the ligand used in WorkBeads 40 TREN is shown in Figure 1. WorkBeads 40 TREN resin can be used for the separation of biomolecules exploiting surface charge to purify proteins, peptides and oligonucleotides. It can also be used in flow through mode to adsorb impurities while letting the target pass through the column (negative chromatography mode).
Get a 20% discount if ordered before 31st March.