The OT-2:<br>Your Personal Pipetting Robot

The OT-2:
Your Personal Pipetting Robot

Get started automating your lab today.

Lab automation has never been easier
Lab automation has
never been easier

The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to
automate many common applications

Watch video
Compare to the Flex

Compare to the Flex

Are you unsure which pipettingrobot is the right one for you? Compare them below.

READ WHAT OUR CUSTOMERS HAVE TO SAY

"The choice landed on Opentrons because we had an urgent need for some relatively simple pipetting tasks in connection with our Covid analysis. The Opentrons robot could be delivered and installed quickly, so the robot was up and running fast. It was also important that the robot had a simple user operation, but at the same time was open-source, so that we could program more complex programs ourselves."

- Søren Jepsen, Bioanalyst in charge of the function Regional Hospital North Jutland, Clinical Biochemistry Department, in Hjørring

JOIN THE LINKEDIN COMMUNITY

JOIN THE LINKEDIN COMMUNITY

Share experiences, tips & tricks and help eachother troubleshoot workflows.

Automate the most time-consuming applications in your lab
PCR setup
Protein Purification
General Liquid Handling
PCR Setup

PCR Setup

Another popular application for automation, especially if you’re prepping a lot of samples. You can use the OT-2 pipetting robot to combine samples and PCR mastermix in the reaction plate that will go into the thermocycler or qPCR machine.

Protein Purification

Protein Purification

With the OT-2 Dual Flow Chromatography (DFC) Protein Purification Workstation, you can automate your small-scale protein purification and proteomics sample processing. This Opentrons Protein Purification Workstation uses Biotage PhyTip columns to perform dual flow chromatography-based protein purification.

General Liquid Handling

General Liquid Handling

The quickest way to save time in the lab. You can easily automate a range of basic liquid handling tasks on the OT-2 pipetting robot, including tube and microplate filling, serial dilution, cherry picking, normalization and sample pooling. Choose from a range of verified protocols in our Protocol Library, create your own, or ask our team to create one for you.

Small footprint, Big impact

Fits on half a standard lab bench

Performs like robots costing 10x more

Metric (width, depth, height)63 cm x 57 cm x 66 cm
Accuracy15% ved 1 μL
Imperial (width, depth, height)25 in x 22.5 in x 26 in
Precision5% at 1 μL
Getting Started

Everything you need to automate your lab, including support at every step

Demo the OT-2

Demo the OT-2

Get a virtual or physical demo of the OT-2 and get answers about your specific application

Order the OT-2

Order the OT-2

Bundle your OT-2 with the pipettes, hardware and labware you need

Receive and unbox your OT-2

Receive and unbox your OT-2

Easy to unbox and set up yourself using our online guide

Custom or urgent support?

Custom or urgent support?

Contact us for onsite installation, custom protocol development and more!

Use what you have,
add what you need

Choose your hardware and pipettes

11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes

Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available

Use your existing labware and reagents

Reagent and consumables agnostic

Compatible with any ANSI/SLAS-compliant labware

MODUL-BASERET HARDWARE

MODUL-BASERET HARDWARE

Expand your robot's capabilities with replaceable on-deck modules - including a thermocycler, heating / cooling block, and magnetic bead-based chemistry block.

Choose the protocol method that’s right for you

A protocol is the code that runs the robot through your experiment. Opentrons have built different ways to create a protocol that are accessible for everyone – from the automation rookie to the experienced Python coder

Custom Protocol

Custom Protocol

Best for users that want a unique or complex protocol - fast

Get our experts to create a protocol optimized for your experiment

Protocol Library

Protocol Library

Best for new users looking to gain experience

Browse well-documented, open-source protocols

Protocol Designer

Protocol Designer

Best for users who want to create a new protocol - without having to code

Use Opentrons' intuitive drag-and-drop interface to create new protocols

Python Protokol API

Python Protokol API

Kontakt os for at få din robot installeret hos dig, for kundespecifikke protokoller og meget mere!

Space for 2 pipettes

Reliable liquid transfers from 1–1000 µL with Opentrons' swappable single- and 8-channel pipettes

ot2 pipettes single

ot2 pipettes single

SINGLE-CHANNEL

P20
P300
P1000

ot2 pipettes 8 channel

ot2 pipettes 8 channel

8-CHANNEL

P20
P300

ot2 pipettes

Tech specs

ot2 tech specs

Dimensions
Dimensions

63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench.

Layout
Layout

SPECSOT2 1

Weight
Weight

48 kg or 105.8 lb

Pipette configurations
Pipette configurations

Single- and 8-channel pipetting.
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes.
Pipettes are easily interchangeable.

Pipette volumes and specs
Time to fill 96-well plate
Time to fill 96-well plate

time to fill

Single-channel accuracy and precision
Single-channel accuracy and precision

single channel

8-channel accuracy and precision
8-channel accuracy and precision

8 channel

Deck
Deck

11 deck slots enable countless configurations.
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.

Connectivity
Connectivity

WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0

Video
Video

Integrated video camera (Included for future or custom functionality)

Audio
Audio

Integrated audio system (Included for future or custom functionality)

Power requirements
Power requirements

Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article.

Frame composition
Frame composition

Rigid steel and CNC aluminum design.

Operating temperature and relative humidity
Operating temperature and relative humidity

Recommended 20–25 °C
Recommended 40–60% RH

Sterility
Sterility

Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment. Add the Opentrons HEPA Module, or add your own UV lights.

Viscosity
Viscosity

For best practices on automating viscous liquid handling, review our application note.
Viscous Liquid Handling Automation using Opentrons OT-2 ›

API compatibility
API compatibility

Interfaces with any product that has an API.
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.

Interface
Interface

Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.

Tip compatibility
Tip compatibility

Use Opentrons Tips and Opentrons Filter Tips.
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility
Labware compatibility

Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.

Warranty
Warranty

One year warranty against manufacturer defects.
Stress tested to last a minimum of 3 years of full-time use.

Certifications
Certifications

Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)

Example Commands
Example Commands

Tip Handling
Pick up tip
Drop tip
Return tip
New tip (always, never, once)
More…

Liquid Control
Aspirate
Dispense
Aspirate speed
Dispense speed
Blowout
Touch tip
Mix
Air gap

Utility
Move to
Delay
User-specified pause
Homing
Comment

Transfer
Basic transfer
Volumes > Pipette Volume
Distribute
Consolidate

Variable Settings
Speed
Volume
Count

Other
Dispose

What's in the box
What's in the box

BOX

  • OT-2 robot
  • Calibration block
  • Removable block
  • Trash container
  • Quickstart guide
  • Power supply and regional IEC cable
  • Ethernet cable
  • Ethernet-to-USB dongle
  • Tool kit
Compare to the Flex

Compare to the Flex

Are you unsure which pipettingrobot is the right one for you? Compare them below.

Introduction to the OT-2
Introduction to the OT-2
Watch video
YouTube video

Unboxing the OT-2

YouTube video

Unlocking the OT-2

YouTube video

Setting up the Opentrons app

YouTube video

Attaching pipettes to the OT-2

YouTube video

Deck calibration on the OT-2

YouTube video

Tip Length and Pipette Offset Calibration on the OT-2

YouTube video

Running a protocol on the OT-2

YouTube video

How the Opentrons platform works

YouTube video

Setting up your OT-2

YouTube video

Custom modular Hardware

Frequently Asked Questions
What kind of Labware is compatible with the OT-2?

Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.

Do I need to know how to code to use the OT-2?

You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.

What does an OT-2 protocol look like?

You can find our community’s collection of OT-2 protocols within Opentrons' Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!

If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Does the OT-2 integrate with third party lab equipment?

Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.

Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of Mikrolab - Frisenette.

What certifications does the OT-2 have?

The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated

Can the OT-2 be used in a biological safety cabinet?

Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.

Free demo

Free demo

Schedule a demo with our Opentrons experts, no strings attached