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Rhodamine [4-TB/Rhodamine/101], flame-sealed into triangular cells for quantum yield and spectral correction.
Fluorescent reference materials Molecular fluorescence spec tros co py is a sensitive and often se lec tive technique. Unlike ab sorp tion spec tro pho tom e try it is not an ab so lute technique: in stru ments therefore require calibration before every se ries of measurements. This may be achieved using a stable reference ma te ri al, which should absorb and emit at similar wave lengths to the samples of interest. Use of the general purpose fluorescent reference material set type 6BF enables the day to day sta bil i ty of instruments to be measured. The 6BF reference ma te ri als are not standards with absolute values, but a set of six relatively stable fluorescent materials in a polymethylmethacrylate matrix with which the stability and precision of the in stru ment can be monitored. Four blocks exhibit broad band spectra which cover the normally used UV and visible region of the spectrum, with considerable overlap. Two blocks contain materials suitable for il lus trat ing the selectivity of the tech nique as well as checking instrument resolution and wave length cal i bra tion. The ma te ri als have the following ad van tag es: Stability: no degradation, no evap o ra tion. Safety: no chemicals to mix. Robust: unbreakable, easy to store and use.